Figure 1

Effects of P61A6 on growth of lung cancer cells. A. H23, H1703 and H358 cells were treated with DMSO or the indicated concentrations of P61A6 for 72 hours and surviving cell numbers were counted with cell counting kit, normalized to the control cells without treatment. B. H358 cells under normal growth conditions (medium with 10% FBS) or nutrient-starved conditions (medium with 0.5% FBS) were treated with GGTI P61A6 for 72 hours and cell proliferation relative to the DMSO control (100%) is plotted. C. H358 cells were seeded at a cell density of 20,000 cells/well in 6-well culture dishes in 0.4% agar over a 0.8% bottom agar layer. Various concentrations of P61A6 or DMSO were included in the top layer of cells. Cultures were re-fed and treated with P61A6 or DMSO once weekly (14 days of incubation in total). Colonies were stained with 1 mg/ml MTT for 1 hour and scanned. This experiment was carried out twice with similar results.