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Table 1 BAL fluid cytokine levels in mice 16 weeks post-XRT

From: Radiation mitigating properties of the lignan component in flaxseed

  Cytokine values (pg/ml) (mean ± SEM)
  FGF-basic IL-5 IL-6 IL-12 (p40/p70) KC MCP-1 VEGF
No XRT (13.5 Gy)
 0% FLC ND1 4.6 ± 0.6 3.7 ± 0.2 3.2 ± 1.2 ND1 3.3 ± 0.9 10.9 ± 1.5
 10 % FLC ND1 2.3 ± 0.6a 2.6 ± 0.8 0.2 ± 0.1 ND1 3.9 ± 1.0 8.5 ± 1.8
 20% FLC ND1 2.1 ± 0.3a 2.4 ± 0.6a ND1 ND1 2.7 ± 0.8 5.7 ± 0.5a
XRT (+24 Hours)
 0% FLC + 13.5 Gy 52.5 ± 5.5b 15.3 ± 5.3 129.0 ± 86.6 114.5 ± 24.5b 261.8 ± 52.6 77.9 ± 39.7 47.2 ± 5.0b
 10% FLC + 13.5 Gy 55.3 ± 5.4 5.2 ± 0.9 ND1 73.4 ± 25.9 18.1 ± 4.4 27.2 ± 0.7 52.1 ± 9.3
 20% FLC + 13.5 Gy 36.0 ± 6.4 10.7 ± 4.5 49.1 ± 22.3 91.1 ± 21.4 ND1 29.1 ± 0.9 38.4 ± 3.3
XRT (+48 Hours)
 0% FLC + 13.5 Gy 52.5 ± 5.5 15.3 ± 5.3 129.0 ± 86.6 114.5 ± 24.5 261.8 ± 52.6 77.9 ± 39.7 47.2 ± 5.0
 10% FLC + 13.5 Gy 61.7 ± 4.8 2.1 ± 0.3c 38.4 ± 12.8 6.7 ± 2.3c ND1 26.8 ± 1.5 7.2 ± 3.0b
 20% FLC + 13.5 Gy 37.5 ± 8.6 7.3 ± 3.6 132.3 ± 75.0 83.7 ± 34.6 ND1 28.2 ± 1.7 39.7 ± 11.8
XRT (+72 Hours)
 0% FLC + 13.5 Gy 52.5 ± 5.5 15.3 ± 5.3 129.0 ± 86.6 114.5 ± 24.5 261.8 ± 52.6 77.9 ± 39.7 47.2 ± 5.0
 10% FLC + 13.5 Gy 35.6 ± 11.2 13.7 ± 7.6 369.7 ± 241.7 88.1 ± 32.5 425.7 ± 141.9 82.5 ± 49.9 33.0 ± 10.5
 20% FLC + 13.5 Gy 34.8 ± 8.8 2.3 ± 0.8c ND1 22.7 ± 11.8c ND1 23.1 ± 0.7 30.2 ± 9.5
  1. 1ND = Not Detectable in Assay.
  2. a = p≤0.05 for non-irradiated 0% FLC vs. non-irradiated 10% FLC and 20% FLC.
  3. b = p<0.01 for non-irradiated 0% FLC vs. irradiated 0% FLC.
  4. c = p<0.05 for irradiated 0% FLC vs. irradiated 10% FLC and 20% FLC.
  5. Mice were pre-fed 0% FLC for 72 hours prior to single fraction thoracic X-ray radiation therapy (13.5 Gy). Following XRT exposure, mouse cohorts (n=15) were fed 10% FLC or 20% FLC diets initiated 24, 48, or 72 hours post-XRT. Control-fed mouse cohorts remained on 0% FLC diet throughout the course of the study. Mice were sacrificed at 16 weeks post-XRT and bronchoalveolar lavage (BAL) fluid was collected. BAL cytokine analysis was performed and data is represented as mean ± SEM. a= p≤0.05 for non-irradiated 0% FLC vs. non-irradiated 10% FLC and 20% FLC, b= p<0.01 for non-irradiated 0% FLC vs. irradiated 0% FLC, and c= p<0.05 for irradiated 0% FLC vs. irradiated 10% FLC and 20% FLC.