Evaluation of blood oxygenation levels and lung injury in mice 16 weeks post-XRT. Mice were pre-fed 0% FLC for 72 hours prior to single fraction thoracic X-ray radiation therapy (13.5 Gy). Following XRT exposure, mouse cohorts (n=15) were fed 10% FLC or 20% FLC diets initiated 24, 48, or 72 hours post-XRT. Control-fed mouse cohorts remained on 0% FLC diet throughout the course of the study. Mice were sacrificed at 16 weeks post-XRT. Panel A: Pulse oximetry analysis was performed prior to sacrifice at 16 weeks post-XRT. Data is represented as mean ± SEM. *p< 0.01 for irradiated 0% FLC vs. irradiated 10% FLC (+24 Hours). Panel B: BAL protein levels were determined at 16 weeks post-XRT. Data is represented as mean ± SEM. *p< 0.01 for irradiated 0% FLC vs. irradiated 10% and 20% FLC.