Representative HE-stained sections and immunohistochemical staining for ACY1, SQSTM1, and GPC3 (×200). (A) Typical HE-stained sections and immunostaining for ACY1, SQSTM1, and GPC3 are shown for LGDN, HGDN, WDHCC, and MDHCC. P, positive immunostaining; N, negative immunostaining. (B) Immunohistochemical expression of ACY1, SQSTM1, and GPC3 in LGDN, HGDN, WDHCC, and MDHCC. A box and whisker plot (whiskers: 10–90%) of IOD for each marker was obtained from the tissue microarrays. Mann–Whitney tests showed a significant difference between WDHCC (32 lesions) and MDHCC (19 lesions) compared with HGDN (21 lesions).