Analysis of expression after DNMT1 knock-down. (A) DNMT1 was depleted by siRNA in HEC-1A and ECC1 cells and the efficacy of the knock-down was analyzed by RT-PCR. (B) Effect of DNMT1 depletion on the expression of the indicated gene products as analyzed by RT-PCR. siGFP was used as control. (C) Cells were treated as described above and cell lysates were prepared for Western blot analysis. Primary antibodies to the indicated proteins were used followed by peroxidase conjugated Goat anti mouse IgG and ECL detection. Representative blots from n = 2 experiments are shown.