Figure 3

Rsv abrogates TMZ-induced G2 cell cycle arrest accompanied by decrease of Cdk1(Y15). (A) Cell cycle distribution of U87 cells treated with Rsv 30 μM, TMZ 100 μM or cotreatment T100+R30 for 48 h (left) TMZ for 72 h, or TMZ 48 h followed by 24 h in complete drug-free medium, or 24 h of Rsv 30 μM (right). Top: representative histograms of flow cytometry; numbers between the lines indicates the percentage of cells in each phase of cell cycle, as indicated; ^a p<0.001 and ^c p<0.05 in relation to control without drug; (B) DNA damage index measured by the comet assay in cells treated as in (a) for 20 and 48 h. top panel – representative images of nuclei after comet assay; scale bar: 10 μm; bottom – quantification of DNA damage index; (C and D) U87 cells were treated with Rsv 30 μM, TMZ 100 μM or cotreatment T100+R30 for 24 or 48 h, followed by western blotting using the indicated antibodies. Numbers indicate the band intensity in relation to control. LC – loading control (stained membrane); ND – not detected; * p<0.05, ** p<0.01 and *** p<0.001 in relation to control of the same time.