Figure 2

Autophagy is not involved in the toxicity of RT cotreatment. (A) U87 cells were pre-incubated with 3MA (2 mM) or vehicle (PBS) for 1 h before treatment with Rsv 30 μM, TMZ 100 μM or RT for 48 h. Upper line – LC3-GFP transfected cells; number = percentage of LC3-GFP-positive cells; scale bar: 10 μm; second line - representative images of AO-marked cells; scale bar: 20 μm; third and fourth lines – flow cytometry of AO-marked cells; numbers in the quadrants refer to average number of events (black) or mean intensity of red fluorescence (Red) ± SEM; ^a p<0.05, ^b p<0.01 and ^c p<0.001 in relation to control; ^d p<0.001 in relation to TMZ alone; * p<0.05 and *** p<0.001 in relation to treatments with the vehicle; (B) number of cells after pre-treatment with 3MA followed by treatment as in (a); control was considered 100%; * p>0.05; ** p>0.01, and # p>0.05 in relation to the same treatment without 3MA.