Figure 4
HIF-1α silencing in HCC cells inhibits SNAI1-mediated EMT and invasion under CoCl 2 treatment. (A) mRNA levels of HIF-1α, SNAI1, E-cadherin, N-cadherin and Vimentin were determined in CoCl2-treated SMMC-7721 cells infected with Ad-scrambled or Ad-shHIF1α (10 MOI) by qRT-PCR. GAPDH was used as endogenous reference and mRNA level of untreated SMMC-7721cells was used as standard. Data were shown as mean ± SD of three independent experiments. (B) Protein levels of E-cadherin, N-cadherin and Vimentin in CoCl2-treated SMMC-7721 cells infected with Ad-scrambled or Ad-shHIF1a was determined by immunoblotting. β-actin was used as endogenous reference. (C) The numbers of invasive and migrating CoCl2-treated SMMC-7721 cells infected with Ad-scrambled or Ad-shHIF1α was calculated with crystal violet staining. The average numbers of ten random microscopic fields (×400) was recorded in each experiment. Data was shown as mean ± SD of three independent experiments. Representative images of each group were shown.