Figure 3
Overexpression of PRAF3 enhances the activity of caspase-8 and caspase-9 in ESCC cells. 1.0 × 106 Eca109 and TE-1 cells were infected with Ad.PRAF3 (or Ad.Null) at a MOI of 100. The activity and expression of caspase-8 and caspase-9 were detected at 72 hr post-infection. (A, B) The activity of caspase-8 and caspase-9 were detected by ApoAlert caspase colorimetric and fluorescent assays, respectively. The activity of the Ad.Null-infected cells at 0 hr was defined as 1. Histogram showed the activity of caspase-8 (A) and caspase-9 (B) in Ad.PRAF3-infected and Ad.Null-infected ESCC cells. (C, D) The expression of caspase-8 and caspase-9 were detected by western blot and normalized to that of β-actin. Photographs showed the western blot products and histograms showed the expression of caspase-8 (C) and caspase-9 (D) in Ad.PRAF3-infected and Ad.Null-infected ESCC cells. Data represent mean ± SEM from 4 independent experiments; *, P < 0.05, **, P < 0.01 by t test.