Figure 1

Characterization of H1650-ER1 cells. (A) mRNA expression of E-cadherin, vimentin, occludin and fibronectin in H1650 and H1650-ER1 cells was measured by quantitative RT-PCR. Fold change expression was normalized with respect to H1650 cells. (B) mRNA expression of Snail, Twist and Zeb1 in H1650 and H1650-ER1 cells was measured by quantitative RT-PCR. Fold change expression was normalized with respect to H1650 cells. Error bars represent s.e.m. (n = 3). (C) Immunofluorescence of H1650 and H1650-ER1 cells stained with DAPI (blue) and antiβ-catenin antibody (green). (D) H1650 and H1650-ER1 cells were seeded on 6 well plates. After 48 hr, a scratch was induced in the confluent cell monolayer. Images were obtained at time t = 0 and after 12 hr (t = 12) to monitor cell migration. Percent cell migration was calculated based on migration of H1650 cells. The error bars represent s.e.m. (n = 3).