Figure 1

TST measurements of Dunning rat CaP aggregates. Comparison of aggregate cohesion by TST shows that aggregates composed of a 4:1 mixture of JHU-3: Rat-2 and AT-2: Rat-2 are significantly more cohesive than those of MLL: Rat-2 (ANOVA, p < 0.0001, Tukey's MCT, JHU-3:AT-2 p < 0.001, JHU-3-MLL p < 0.001, AT-2:MLL p < 0.001). The data set is comprised of n = 20 JHU-3, n = 21 AT-2, and n = 20 MLL aggregates, representing 40, 42, and 40 compressions, respectively (A). Aggregate cohesion is independent of volume. The relationship between σ and aggregate volume was assessed by linear regression analysis. For liquid-like aggregates, σ must be independent of volume. This was confirmed by showing that the correlation coefficient, R², of regression lines for MLL, AT-2, and JHU-3 are 0.23, 0.06, and 0.07, respectively, indicating that σ is independent of volume (B).