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Table 2 Inhibition of PEA hydrolysis in B16

From: The association of N-palmitoylethanolamine with the FAAH inhibitor URB597 impairs melanoma growth through a supra-additive action

   PEA hydrolysis inhibition (% ± SEM)
   Cell homogenates Intact cells
URB597 10 μM 95 ± 1.0 93 ± 3.7
  1 μM 90 ± 4.1 85 ± 5.2
CAY10402 10 μM 95 ± 6.3 75 ± 4.6
  1 μM 83 ± 5.2 65 ± 5.1
MAFP 10 μM 86 ± 6.3 70 ± 4.2
  1 μM 88 ± 3.1 75 ± 3.0
CAY10499 10 μM 100 ± 7.5 80 ± 4.4
  1 μM 91 ± 3.9 64 ± 4.9
CCP 10 μM 6 ± 4.2 26 ± 6.8
  1 μM 4 ± 5.6 26 ± 7.0
  1. FAAH inhibitors (URB597, CAY10402), dual FAAH/MAGL inhibitors (MAFP, CAY10499) and NAAA inhibitor (CCP) were tested at concentrations of 1 and 10 μM on cell homogenates (25 μg protein, pH 7.4) and on living cells (105 cells/well, seeded 24 h before) in culture medium. Data are the mean of three experiments and are expressed as percentage of the control containing vehicle instead of the inhibitors