The association of N-palmitoylethanolamine with the FAAH inhibitor URB597 impairs melanoma growth through a supra-additive action

Table 2 Inhibition of PEA hydrolysis in B16

		PEA hydrolysis inhibition (% ± SEM)
		Cell homogenates	Intact cells
URB597	10 μM	*95 ± 1.0*	*93 ± 3.7*
	1 μM	*90 ± 4.1*	*85 ± 5.2*
CAY10402	10 μM	*95 ± 6.3*	*75 ± 4.6*
	1 μM	*83 ± 5.2*	*65 ± 5.1*
MAFP	10 μM	*86 ± 6.3*	*70 ± 4.2*
	1 μM	*88 ± 3.1*	*75 ± 3.0*
CAY10499	10 μM	*100 ± 7.5*	*80 ± 4.4*
	1 μM	*91 ± 3.9*	*64 ± 4.9*
CCP	10 μM	*6 ± 4.2*	*26 ± 6.8*
	1 μM	*4 ± 5.6*	*26 ± 7.0*

FAAH inhibitors (URB597, CAY10402), dual FAAH/MAGL inhibitors (MAFP, CAY10499) and NAAA inhibitor (CCP) were tested at concentrations of 1 and 10 μM on cell homogenates (25 μg protein, pH 7.4) and on living cells (10⁵ cells/well, seeded 24 h before) in culture medium. Data are the mean of three experiments and are expressed as percentage of the control containing vehicle instead of the inhibitors

ISSN: 1471-2407