Skip to main content

Table 1 Targeted protein analysis of A2780 and A2780cis cells.

From: EMT transcription factors snail and slug directly contribute to cisplatin resistance in ovarian cancer

Protein

Peptide

Fragment Change

Fold

Mean Peak Area

CV

    

Sensitive

Resistant

Sensitive

Resistant

E- cadherin

 

b8

-1.18

5.82E+04

3.22E+04

0.01

0.13

 

GPFPKNLVQJK

b9

-1.55

1.57E+04

1.01E+06

0.07

0.08

  

b10

-1.63

3.33E+04

2.05E+04

0.03

0.19

  

y8

-4.48

2.10E+04

5.02E+03

0.09

0.10

 

VFYSITGQADTPPVGVFIIER

y9

-4.16

8.25E+04

1.99E+04

0.02

0.12

  

y10

-4.01

8.45E+04

2.11E+04

0.07

0.11

  

y6

1.15

4.78E+06

2.52E+06

0.01

0.01

 

EYQDLLNVK

y7

1.14

2.19E+06

2.50E+06

0.02

0.01

Vimentin

 

y8

1.11

1.27E+05

1.14E+05

0.02

0.05

  

y6

1.01

4.58E+06

4.62E+06

0.02

0.03

 

ILLAELEQLK

y7

1.08

7.34E+06

7.94E+06

0.04

0.05

  

y8

1.10

1.11E+07

1.22E+07

0.04

0.04

  

y9

1.08

1.35E+06

1.47E+06

0.04

0.04

  

b3

1.25

6.00E+04

7.50E+04

0.14

0.03

Snail

SFLVR

b4

1.21

7.38E+04

8.89E+04

0.03

0.07

  

y3

1.29

2.14E+04

2.76E+04

0.16

0.04

  

b3

1.10

1.92E+04

2.10E+04

0.18

0.14

Slug

HFNASK

b5

1.27

2.40E+05

3.06E+05

0.06

0.16

  

b10

1.05

9.96E+04

1.04E+05

0.13

0.07

 

VSPPPPSDTSSK

b11

1.19

4.47E+04

5.34E+04

0.07

0.05

  

y9

1.24

4.00E+04

4.96E+04

0.07

0.06

  1. Summary of SRM-MS quantification results for E-Cadherin, Vimentin, Snail, and Slug. Mean peak area and coefficient of variation (CV) for each peptide fragment ion were derived from peak areas measured in three replicate assays of each of the sensitive and resistant cell line samples. Transitions with CV > 0.20 in either sample are not shown