Figure 6

Reduction in both tumor sphere size and SOX2 expression following PLK1 mRNA inhibition by RNAi. (A) Bar graph quantifies the average tumor sphere diameter for the three different conditions: Daoy undergoing no treatment, transfected with non-targeting shRNA (shNTC), or shRNA targeting PLK1 (shPLK1 1325). Error bars represent SEM. Shown below the bar graph are the representative pictures of Daoy primary tumor spheres. The diameter of the tumor spheres was measured using QCapture Pro software from saved images. Magnification 4×. (B) Bar graph quantifying the levels of SOX2 mRNA expression in Daoy cells following shRNA transfection. Red bars represent mRNA levels in cells grown in standard adherent monolayer conditions. Blue bars depict mRNA levels for cells grown in the non-adherent tumor sphere conditions. Error bars represent SEM