Figure 5

Pretreatment with BI 2536 enhances radiation sensitivity in medulloblastoma cell lines. (A) Line graph plotting the surviving fraction of Daoy cells given different radiation doses following 24 hours of treatment with BI 2536 or the vehicle control DMSO. Blue line and symbols represent DMSO treated cells. Green line and symbols represent cells treated with 3 nM BI 2536. Red line and symbols represent cells treated with 5 nM BI 2536. (B) Bar graph showing the quantification of the surviving fraction following 2 Gray (Gy) irradiation. (C) Sensitizer enhancement ratio (SER) for Daoy cells. Blue bars are calculated using a survival fraction of 10% (SF0.1) and green bars are calculated using a survival fraction of 50% (SF0.5). (D) Line graph plotting the surviving fraction of ONS-76 cells. Blue line and symbols represent DMSO treated cells. Green line and symbols represent cells treated with 3.75 nM BI 2536. Red line and symbols represent cells treated with 7.5 nM BI 2536. (E) Bar graph quantifying the surviving fraction at the 2 Gray (Gy) level of irradiation for ONS-76 cells. Error bars represent SEM. (F) Bar graph depicting the sensitizer enhancement ratio in ONS-76 cells. Blue bars are calculated using a survival fraction of 10% (SF0.1) and green bars are calculated using a survival fraction of 50% (SF0.5)