Figure 4

Inhibition of PLK1 with BI 2536 decreases cell growth and increases apoptosis. (A) Decrease in the relative cell number of two medulloblastoma cell lines, Daoy and ONS-76, through a wide range of BI 2536 concentrations treated for 72 hours. Error bars represent SEM. (B) Quantifying bar graph showing a significant decrease in the ability of two medulloblastoma cell lines in forming colonies following 24 hour treatment with BI 2536. Error bars represent SEM. Shown below are representative pictures of the wells from the colony formation assay. (C) Representative flow cytometry plots for Daoy cells stained with Guava Nexin reagent following 24 hours of treatment with BI 2536. Percentages of cells in the bottom left quadrant are live while cells to the right of the vertical line are apoptotic or dead. (D) Bar graph quantifying the percentage of apoptotic/dead cells following Guava Nexin reagent staining. Error bars represent SEM