Copy number analysis of spiked cells isolated from healthy blood via IE/FACS. Twenty (20) isolated (A) LNCaP, (B) PC3 and (C) VCaP cells (in duplicate) and 50 ng of genomic DNA from cell culture subjected to whole genome amplification, and unamplified genomic DNA (600 ng) from cell culture (positive control) were analyzed for copy number aberrations. The log2 ratio value for each BAC clone is plotted on the y-axis. The x-axis represents the genomic position of each BAC clone on the array, with chromosome numbers indicated. Vertical solid lines indicate chromosome boundaries, and vertical red dashed line represents the centromeric region dividing each chromosome into the p- or short arm (to the left of centromere) and the q- or long arm (to the right of the centromere). Arrows indicate high-level gains on X chromosome region containing AR observed in VCaP cells.