Expression of ARF in human retinoblastoma cell lines. (A) Comparison of ARF mRNA level between normal human fetal (FR) and adult retinas (HR 50 and 108), RB1
-/- retinoblastoma cell lines (RB 247, 381, 1021, WERI-Rb1 (WERI), Y79), RB1
+/+ cell lines (RB 3823, 522), and other cell lines (HeLa, SKOV3, HEK-T, SaOS2, OVCAR3) using quantitative real-time PCR. GAPDH was used as an endogenous control. (B) Western Blot analysis of ARF in fetal retina (FR), adult retina (HR 50), RB1
-/- retinoblastoma (RB247, 381, 1021, WERI-Rb1 (WERI), Y79), RB1
+/+ retinoblastoma (RB 3823, 522), and other (HeLa, HEK-293T, OVCAR-3 and SaOS-2) cell lines. *Same cell extracts were added to ensure consistent ARF protein expression/detection between immunoblots. (C) Ratio of relative ARF protein expression (normalized to β-tubulin, relative to FR) to relative ARF mRNA (normalized to GAPDH, relative to FR). (D) Confirmation of full length ARF mRNA expression in fetal retina (FR), human retina (HR 50), retinoblastoma cell lines and other cell lines using RT-PCR.