Figure 5

AKT mediated phosphorylation leads to an increase in BCA2 stability through 14-3-3σ interaction. [A] Immunoblot of equal amounts of lysates of HEK293T cells co-transfected with wild-type GST-BCA2 or GST-BCA2 S132, 133A along with either dominant negative or constitutively active AKT. [B] Co-immunoprecipitation of wild-type BCA2 and 14-3-3σ in the presence (lane2) or absence (lanes 1, 3) of AKT inhibitor LY294002 and co-immunoprecipitation of S132, 133A BCA2 and 14-3-3σ. [C] Western blot showing the results of a stability assay in the presence of absence of MG-132 on lysates of HEK293T cells which had been tranfected with either wild-type FLAG-BCA2, Xpress-14-3-3σ or both.