Immunohistochemical analysis of tumor antigens in resected tumor tissue from patient 6. Many positively stained pleomorphic giant cells and their processes were shown. Each primary antibody against MAGE-A1, MAGE-A3, and WT-1 was used at a dilution of 1:10 ~ 1:100. Mouse IgG antibody was used as a control. H & E stain; hematoxylin and eosin stain. Magnification ×400.