Figure 5

The tumorigenicity of the transduced U251/brevican-shDNA 2 cells was reduced in nude mice. (A) Photograph of subcutaneous tumors formed by the transduced U251 cells (1), the transduced U251/brevican-mock cells (2) and the transduced U251/ brevican-shDNA 2 cells (3) groups (n=5) at day 28. Bars are 10 mm. (B) The plain MRT and Gd-DTPA-enhanced MRT results for brain sections showed that the tumorigenicity of the transduced U251/brevican-shDNA 2 cells (b, d) was dramatically limited at day 25 (n=3), compared with the transduced U251/brevican-shDNA mock cells (a, c). Also, the diffusion degree of the transduced U251/brevican-mock cells (a, c) was more extensive than the transduced U251/brevican-shDNA 2 cells’ (b, d). TU, the transduced U251 cells; TU-shDNA mock, the transduced U251/brevican-mock cells; TU-shDNA2, transduced U251/brevican-shDNA 2 cells; a, b, the plain MRT results; c, d, Gd-DTPA-enhanced MRT results. * P < 0.05; ** P < 0.01.