Figure 1
From: Up-regulated expression of l-caldesmon associated with malignancy of colorectal cancer
A 2-DE-based comparative proteome analysis of normal colon mucosa samples and colon tumors obtained from colon cancer patients. (a) Typical 2-DE images of whole proteomes extracted from two different tissues. The protein identified by the white arrow in the enlarged image was overexpressed in colon tumor tissues. MALDI-MS analysis of the protein highlighted in the upper panel unambiguously identified it as caldesmon. (b) Differential expression of the 65 kDa isoform of caldesmon (l-CaD). Two caldesmon isoforms, h-CaD (80 kDa) and l-CaD (65 kDa) were dominantly detected in tissues from colon cancer patients (CPs) by western blot analysis. The level of the l-CaD was significantly increased in colon tumors (T) compared with normal colon mucosa (N).