Figure 6

Restoration of WNT7A does not induce the canonical β-catenin pathway in Jurkat cells. (A) Relative expression levels of AXIN2, MYC, JUN, and FRA-1, determined by qRT-PCR in Jurkat-pLVX or Jurkat-pLVX-WNT7A clone-1 and -2 infected cells. Quantification was calculated by normalizing with Jurkat-pLVX (setting as 1) and utilizing GAPDH and RPL32 as reference genes. The graphic shows medians obtained with both reference genes ± Standard deviations (SD). (B) Relative expression levels of AXIN2, MYC, JUN, and FRA-1, determined by qRT-PCR in Jurkat-pLVX or Jurkat-pLVX-WNT7A clone-1 in the absence or presence of LiCl. Quantification was calculated by normalizing LiCl-treated cells with their corresponding Jurkat-pLVX or Jurkat-pLVX-WNT7A non-treated cells (setting each as 1) and utilizing GAPDH and RPL32 as reference genes. The graphic shows medians obtained with both reference genes ± SD. (C) Western blot analysis detecting β-catenin and β-actin in the presence or absence of LiCl and WNT7a.