Figure 4

WNT7A overexpression in Jurkat cells. Overexpression of WNT7A in Jurkat cells using the lentiviral system was confirmed by qRT-PCR assays. (A) Graphic showing WNT7A relative expression taking GAPDH, RPL32 and RPS18 as constitutive genes in cells infected with pLVX (vector alone) or with pLVX-WNT7A. Infection was carried out in duplicate (pLVX-WNT7A clone 1 and 2). (B) Amplification products of WNT7A and RPS18 were run in 2.0% agarose gels and photodocumented. (C) Western blot assays (from 50 μg total protein) showing the presence of WNT7a protein only in pLVX-WNT7A-infected cells. The magic mark (2 μl) was used as protein marker.