Figure 3

Interaction between SNCG and AR protein regulates androgen-induced transcriptional activity of AR . (A) After culture in androgen-deprived medium, SNCG mRNA expression in LNCaP cells was detected by RT-PCR. (B) LNCaP cells were treated with increasing amounts of androgens (dihydrotestosterone DHT at 0.1, 1.0, 10.0 and 100 nM) and anti-androgens (flutamide Flu at 0.1, 1.0, 10.0 and 100 μM) for 48 h before SNCG mRNA expression was analyzed by quantitative RT-PCR and compared to the levels in the parental LNCaP cells. (C) The co-IP assay indicated that the interaction between SNCG and AR was strengthened by DHT. IgG-precipitated complexes were used as the control. (D) PSA mRNA expression in siSNCG-LNCaP cells was measured after administration with androgen. (E) RT-PCR was used to detect the AR mRNA expression in siSNCG-LNCaP cells with or without DHT administration. (F) Dual-luciferase reporter assays were performed to show the AR transcriptional activity in siSNCG-LNCaP cells with or without DHT administration. ^* P < 0.05, ^** P < 0.01.