Suppression of SNCG in LNCaP Cells inhibits cellular proliferation and induces cell cycle arrest at G1. (A) Top: RT-PCR analysis was used to evaluate SNCG mRNA expression levels in androgen-dependent (LNCaP) and androgen-independent human advanced prostate cancer lines (DU145, PC3 and LNCaP-AI). Bottom: Western blot analysis revealed SNCG protein expression levels in the relevant cell lines. (B) Top: RT-PCR analysis was used to evaluate SNCG mRNA expression in LNCaP cells after transient transfection with various siRNA constructs. Bottom: Western blot analysis showed oligo-166 inhibited SNCG expression in LNCaP cells. (C) CCK-8 assay were performed for evaluation of the LNCaP cellular proliferation. (D) Flow cytometry assay demonstrated that inhibition SNCG expression in LNCaP cell induced cell cycle arrest at G1. These results are representative of three independent experiments. *
P < 0.05, **
P < 0.01.