Inhibition of reductions of IL-18 and IFN-γ in sera and intestinal cells. Blood and intestinal samples were collected from the mice in the prevention when they were killed on days 29, 36 and 43. A,B: The serum levels of IL-18 (A) and IFN-γ (B) were measured in the above mice and a group of 6 healthy control mice. C: The intestinal tissues were sectioned, immunostained with Abs against mouse IL-18 and IFN-γ, respectively, and examined by microscopy. IL-18+ and IFN-γ+ cells were counted in 10 fields. Results are expressed as the mean value ± SD. “*” P < 0.05 and “**” P < 0.001 versus the group fed the control diet, “†” P < 0.05 versus the group fed the control diet and treated with tamoxifen, and “‡” P < 0.05 versus the group fed the Fe-Lf diet.