Skip to main content

Advertisement

Table 1 Immunostaining protocol details listed by antigen

From: Blood vessel hyperpermeability and pathophysiology in human tumour xenograft models of breast cancer: a comparison of ectopic and orthotopic tumours

  CD31 Collagen IV αSMA LYVE-1
NGS blocking incubation time 1 h 1 h 1 h 20 min
Primary antibody dilution 1:200 1:1000 1:1000 1:1000
Secondary antibody incubation time 1 h 30 min 30 min 30 min
ABC reagent incubation time 1 h 30 min 30 min 30 min