Figure 4

Box-plots of PLA2R1 methylations in DNA from blood of healthy individuals (Controls, N = 52) and leukemic patients (Leukemia, N = 32). MS-HRM analyses of amplified PLA2R1 sequences (covering 5`-CpG sites 6–14, -437 bp to −270 bp from exon 1) after isolation and subsequent bisulfite modification of genomic DNA from blood samples were performed using RotorGene Q (Qiagen). Boxes within the plots represent the 25-75th percentiles. Median values are depicted as solid lines. Circles indicate outlier values outside of the 10th and 90th percentiles.