5`-CpG islands and methylation of proximal promoter and down-stream regions of the PLA2R1 gene determined by sequencing of bisulfite modified genomic DNA. (A) PLA2R1 promoter and down-stream regions covering −700 to +1340 bp relative to exon 1 containing four 5`-CpG islands labelled in grey are shown; island-1, 115 bp length (−357 to −243 bp); island-2, 422 bp (+38 to +459); island-3, 157 bp (+512 to +668 bp), and island-4, 155 bp (+837 to +991 bp). Criteria used for 5`-CpG island prediction: island size >100 bp, GC percent >50.0, Minimum observed/expected >0.6. Horizontal black bar depicts the region expanding from −437 to −270 bp relative to exon 1 whose methylation was quantified using MS-HRM analysis. (B) Extents of methylation of distinct 5`-CpG sites in the PLA2R1 promoter and down-stream region of U937 and Jurkat cells as well as PLA2R1 methylations in DNA from blood of 10 healthy individuals and 16 leukemic patients (means ± SDs) determined by sequencing of bisulfite modified genomic DNA. Horizontal black bars show 5`-CpG sites analyzed by MS-HRM analyses.