Figure 3

Curcumin and RL197 decrease expression of Sp-regulated gene products. Downregulation of Sp-regulated gene products by different concentration of curcumin in RKO (A) and SW480 (B) cells, and by different concentrations of RL197 in RKO (C) and SW480 (D) cells was determined by western blot analysis as described in the Materials and Methods. Experiments with Sp proteins (Figure 2) and Sp-regulated gene products were part of the same study and have the same β-actin loading controls.