Figure 1

[ ¹²⁵ I]-Buserelin binding and IP production in MDA cells expressing the WT GnRHR and GnRHR-DesK191 mutant. A. Specific [¹²⁵I]-Buserelin binding to MDA cells transfected with the empty vector (pcDNA3.1), the WT GnRHR and the GnRHR-DesK191 cDNA constructs. B. Inositol phosphate dose–response curves for Buserelin in MDA cells transiently expressing the WT GnRHR and GnRHR-DesK191 mutant. Maximal IP production in cells transfected with the WT GnRHR cDNA was set as 100% and all other values are expressed relative to this. Assays were performed in triplicate incubations and the results shown are the means ± SEM from three independent experiments. *p<0.001; ** p<0.01 vs pcDNA3.1.