Figure 3

Examples from screening of mCRC samples using the c.38 G > T CADMA assay. The wild-type mCRC samples showed more variation in the c.38 G > A CADMA assay compared to any of the other CADMA assays. A. The derivative of the raw melting data (melt curve analysis). No heteroduplexes, which melt between 71 and 74°C, can be observed in any of the wild-type mCRC samples. B. Normalized HRM difference graph. Sample ID 2 deviates more from the wild-type replicates than the standard containing 1% mutant alleles from approximately 79 to 81°C. This should not be interpreted as a c.38 G > T mutation, since no heteroduplexes are present.