Figure 5

MTOR is a target of miR-99a. (A) Sites of miR-99a seed matches in the mTOR 3^′ UTR. (B) Expression of mTOR protein were detected by western blotting assay in RCC cell lines (786-O and OS-RC-2) and normal immortalized renal cell line (HK-2). (C) Expression of mTOR and p-mTOR protein were detected by western blotting assay in RCC cell lines (786-O and OS-RC-2) after 48 hours of transfection with miR-99a or NC. (D) Luciferase constructs were transfected into 786-O and OS-RC-2 cells transduced with miR-99a. Luciferase activity was determined 48 hours after transfection. The ratio of normalized sensor to control luciferase activity is shown. Data are represented as mean ± SD from three independent experiments. **, P < 0.01.