Figure 1

Dual color FISH analysis of PTEN deletion in primary PCa. A) BAC DNA mapping to chromosome 10q23.3 (PTEN) was fluorescently labelled and co-hybridized with fluorescent Centromere 10 control probe to detect PTEN deletion in tumor samples. B) PTEN deletion status of 43 primary PCa samples determined by FISH. C) FISH for PTEN status in representative interphase nuclei of prostate samples. On the left panel, the FISH image shows 1 red signal (10q23.3 locus) and two green signals (centromere 10) per nuclei indicating a PTEN deletion. On the right panel, the FISH image shows two red signals and two green signals in the nuclei indicating no PTEN deletion.