Figure 4

Gene expression of lipid transport proteins and lipid uptake ability. Real-time PCR gene expression profiling of (A) fatty acid transport proteins (FATPs encoded by SLC27A genes) and (B) fatty acid binding proteins (FABPs) of non-transformed prostate epithelial cell line RWPE-1, benign prostatic hyperplasia epithelial cell line BPH-1, and metastatic prostate cancer cell line C4-2. Gene expression levels were normalized to 1 for RWPE-1 cell line and respectively for other cell lines. (C) Oil Red O staining to evaluate the uptake of plasma lipid by RWPE-1, BPH-1, and C4-2 cells after 24 hours of incubation with 50% human plasma.