Figure 5

Downregulation of PCA3 expression does not significantly reduce Akt and Erk1/2 phosphorylation in LNCaP cells. The Akt and Erk1/2 phosphorylation profile before and after transfection of LNCaP cells with either siPCA3 or siScr was investigated by immunoblot analysis using 50 μg of total protein extracts. Akt and Erk1/2 non-phosphorylated proteins were used as controls for normalization of protein loading.