Figure 7

Cl-amidine decreases the growth of MCF10DCIS tumors in a xenograft model of comedo-DCIS. (a) Average tumor volumes after 1, 7, and 14 days of daily IP injections of Cl-amidine (50mg/kg/day) or PBS as vehicle control. MCF10DCIS cells (1 × 10⁶) were injected subcutaneously into female nude (nu/nu) mice (Charles River). After 2 weeks of tumor growth (tumor volumes ~100-200 mm³), mice were randomly treated with intraperitoneal injections of Cl-amidine at 50 mg/kg/day (n = 7) or PBS as a vehicle control (n = 7). Tumor volume was measured weekly by digital caliper, and the differences between tumor volumes were evaluated by the non-parametric Mann–Whitney–Wilcoxon test (MWW). Results are reported as mean ± SD (Day 14, p = 0.002). (b) PAS stained sections (10X, scale bar = 200 μm) from representative treated (I) and control tumors (II), arrows in (I) show an intact basement membrane (BM), while the arrow in (II) shows breaching of the BM with infiltrating leukocytes. (c) BM integrity was scored on a scale from 1–3 by S.M., with values being expressed as the mean ± SD. The treated tumors have a lower score, indicating a higher level of BM integrity (* p < 0.05 MWW).