Table 3 Antibodies used for immunohistochemical analysis
Antigen | Manufacturer | Mono-/polyclonal | Staining | Positive control | Blocking | Concentration |
|---|---|---|---|---|---|---|
phospho-Smad1 (Ser463/465) | Cell signaling | polyclonal rabbit IgG | nucleus | colon | no | 1:200 |
/Smad5 (Ser463/465) | ||||||
/Smad8 (Ser426/428) | ||||||
phospho-Smad2 (Ser465/467) | Cell signaling | monoclonal rabbit IgG1 | nucleus | kidney | 10% normal goat serum, 30’ | 1:50 |
endoglin / CD105 | R&D systems | polyclonal goat IgG | cytoplasmic | tonsil | no | 1:800 |