Table 2 Primer sets used for quantitative RT-PCR analysis
Gene symbol | alias | forward primer(5'to 3') | reverse primer(5'to 3') |
|---|---|---|---|
BMP2 | ACGAGGTCCTGAGCGAGTTC | GAAGCTCTGAGGTGATAA | |
BMP4 | CACCTCATCACACGACTAC | GGCATGGTTGGTTGAGTTG | |
BMP6 | GAGTCGTAATCGCTCTACCC | GTGAACCAAGGTCTGCACAA | |
BMP7 | CCAGAACCGCTCCAAGAC | GTTGGTGGCGTTCATGTAG | |
TGFB1 | CAGAAATACAGCAACAATTCC | CTGAAGCAATAGTTGGTGTC | |
TGFB2 | CCAAAGGGTACAATGCCAAC | CAGATGCTTCTGGATTTATGGTATT | |
TGFB3 | TAAGCGGAATGAGCAGAGG | CCACGCCTTTGAATTTGATTT | |
ACVRL1 | ALK1 | CTGGACATCGGCAACAAC | ACACCACCTTCTTCATGTC |
ACVR1 | ALK2 | TGGCTTCCACGTCTACCA | GCGAACACTACAGAGAGAA |
BMPR1A | ALK3 | TATGGAGAAGTATGGATGGG | GAACCTGTACCTTTAATGTCT |
TGFBR1 | ALK5 | ATTACCAACTGCCTTATTATGA | CATTACTCTCAAGGCTTCAC |
BMPR2 | TGGGATAGGTGAGAGTAGA | GAGGGAGGAGTGGTAGTT | |
TGFBR2 | ATGGAGGCCCAGAAAGATG | GACTGCACCGTTGTTGTCAG |