Subpopulation | Cells | Tumors/injections |
|---|
| | | HeLa | SiHa | Ca Ski | C-4 I |
|---|
| | 1 × 103
| 2/6 | 3/6 | 0/6 | 0/6 |
Spheroid cells | 1 × 104
| 4/6 | 4/6 | 3/6 | 2/6 |
| | 1 × 105
| 5/6 | 4/6 | 4/6 | 4/6 |
| | 1 × 105
| 0/6 | 0/6 | 0/6 | 0/6 |
| | 5 × 105
| 1/6 | 1/6 | 0/6 | 0/6 |
| | 1 × 106
| 5/6 | 2/6 | 0/6 | 0/6 |
- Tumor formation ability of spheroids. Cells were grown in serum-free medium (SFM) as nonadherent cervospheres and assayed for the ability to form tumors after s.c. injection on nu-nu mice at 1 × 103, 1 × 104, and 1 × 105 cells per injection. At the same time, adherent cells were assayed at 1 × 105, 5 × 105, and 1 × 106 cells per injection. The number of tumors that formed and the number of injections performed are indicated for each condition
