Table 1 Primer sequences with corresponding annealing temperatures, efficiencies, and product length in basepairs (bp) used for realtime PCR

GAPDH

5′;-TGGTATCGTGGAAGGACTCA-3′;

1.93

67

132

5′;-CCAGTAGAGGCAGGGATGAT-3′;

DEFA1/3

5′;-ATGAGGACCCTCGCCATCCTTGCT-3′;

2.17

69

285

5′;-TCAGCAGCAGAATGCCCAGCGTCTTCCC-3′;

DEFA4

5′;-GTCTGCTCTTGCAGATTAGTATTCTG-3′;

1.98

69

105

5′;-TTAATCGACACGCGTGCAGCAGTAT-3′;