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Table 4 Verification of overall variability and variability of the EP scores, C q values, and normalized ΔC q values at the molecular-pathological laboratory at the Charité

From: Decentral gene expression analysis: analytical validation of the Endopredict genomic multianalyte breast cancer prognosis test

  Standard deviations
  Cq ΔCq EP
variables    
 day 0.034 0.032 0.093
 day time 0.062 0.073 0.074
 sample position <0.001 0.016 <0.001
 reagent lot 0.182 0.031 <0.001
 operator 0.035 0.073 0.041
total variable noise 0.198 0.114 0.125
replicate noise 0.131 0.085 0.126
total noise 0.238 0.142 0.178
  1. Different operating conditions (variable noise, replicate noise and total noise) are indicated as standard deviations of the Cq values, ΔCq values, and the EP scores.