Skip to main content

Table 2 Overall variabilities and variabilities of the EP scores, C q values, and normalized ΔC q values of all genes

From: Decentral gene expression analysis: analytical validation of the Endopredict genomic multianalyte breast cancer prognosis test

  Standard deviations
  Cq ΔCq EP
variables    
 day 0.024 0.013 0.006
 day time < 0.001 0.016 <0.001
 PCR instrument 0.027 0.009 0.037
 sample position 0.028 0.015 <0.001
 plate lot 0.014 0.011 <0.001
 reagent lot 0.085 0.027 <0.001
 operator 0.003 0.014 0.043
total variable noise 0.097 0.042 0.057
replicate noise 0.176 0.111 0.136
total noise 0.201 0.119 0.147
  1. Different operating conditions (variable noise, replicate noise, and total noise) are indicated as standard deviations of the Cq values, ΔCq values, and the EP scores.