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Table 2 Overall variabilities and variabilities of the EP scores, C q values, and normalized ΔC q values of all genes

From: Decentral gene expression analysis: analytical validation of the Endopredict genomic multianalyte breast cancer prognosis test

 

Standard deviations

 

Cq

ΔCq

EP

variables

   

 day

0.024

0.013

0.006

 day time

< 0.001

0.016

<0.001

 PCR instrument

0.027

0.009

0.037

 sample position

0.028

0.015

<0.001

 plate lot

0.014

0.011

<0.001

 reagent lot

0.085

0.027

<0.001

 operator

0.003

0.014

0.043

total variable noise

0.097

0.042

0.057

replicate noise

0.176

0.111

0.136

total noise

0.201

0.119

0.147

  1. Different operating conditions (variable noise, replicate noise, and total noise) are indicated as standard deviations of the Cq values, ΔCq values, and the EP scores.
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BMC Cancer

ISSN: 1471-2407

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