Figure 5
From: Novel anti-HER2 monoclonal antibodies: synergy and antagonism with tumor necrosis factor-α
Anti-HER2 antibodies exhibit enhanced anti-growth effect in the presence of tumor necrosis factor-α in SK-BR-3 cells. (A) SK-BR-3 cells were plated onto 96-well plates and incubated at 37°C with 5% CO2. After 2 h of incubation, antibodies (0–10 μg/ml) were added, and cells were incubated for 6 days. (B) Antibodies (0–10 μg/ml) were added as described in (A), and cells were incubated for 4 h prior to TNF-α (1000 U/ml) addition to the culture medium. Cells amount was determined by Sulforhodamine B assay after 6 days of treatment. T. Bars, SD; * p < 0.006, ** p < 0.0005, (A, B).