Figure 5

Effect of miR-221 overexpression on the expression of specific genes. MDA-MB-231 cells were transfected with si-Slug molecule alone, si-Slug in combination with miR-221 mimic or a non-relevant miR (miR-Scr) mimic, a non-relevant siRNA (si-Scr) in combination with miR-Scr mimic. (A) Slug, miR-221, E-cadherin, ERα, TRPS1 expression was determined at RNA level, and revealed by quantitative RT-PCR analysis. RT-PCR results were calculated using the ΔΔCt method. Data are represented as fold change respect to control sample (Ctr). Results represent means ± SEM of three independent experiments. p-values ≤ 0.05 were considered statistically significant. (B) Slug, ERα, Vimentin, and E-cadherin expression was determined at protein level, and revealed by Western Blot. Ten micrograms of whole cell lysates were assayed on a 12% SDS-PAGE, and the proteins were visualized using Supersignal Femto Substrate (Pierce). IP3K was used as loading control.