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Figure 4 | BMC Cancer

Figure 4

From: Evidence for different mechanisms of ‘unhooking’ for melphalan and cisplatin-induced DNA interstrand cross-links in vitroand in clinical acquired resistant tumour samples

Figure 4

Effect of gemcitabine on the unhooking of cisplatin or melphalan-induced ICLs in A549 cells. A: Cells were treated for 1 hour with 150 μM cisplatin either alone (blue) or in the presence of 3nM gemcitabine (green) after which the drugs were removed and replaced with drug free media, or media containing 3nM gemcitabine. Samples were taken at different times of post-incubation and ICLs measured using the comet assay. Data are the mean ± s.d. from at least three independent experiments. B: Cells were treated for 1 hour with 50 μM melphalan either alone (red) or in the presence of 3nM gemcitabine (green) after which the drugs were removed and replaced with drug free media, or media containing 3 nM gemcitabine. Samples were taken at different times of post-incubation and ICLs measured using the comet assay. Data are the mean ± s.d. from at least three independent experiments.

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BMC Cancer

ISSN: 1471-2407

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