Figure 2

DNA ICL and γ-H2AX foci formation in A549 cells following treatment with either cisplatin or melphalan. A: Representative comet images from A549 cells either untreated, or treated with 50 μM melphalan for 1 hour. Drug-treated samples shown were following a subsequent 16 hours incubation in drug free medium. B: Cells were treated for 1 hour with either 150 μM cisplatin (blue) or 50 μM melphalan (red) after which the drug was removed and replaced with drug free media. Samples were taken at different times of post-incubation and ICLs measured using the comet assay. Data are the mean ± s.d. from at least three independent experiments. C: Representative A549 cells showing γ-H2AX foci following treatment with cisplatin at 5 μM followed by post-incubation in drug free medium for the times shown. D: Cells were treated with either 2 μM melphalan (red) or 5 μM cisplatin (blue) for 1 hour after which the drug was removed and cells incubated at 37°C in drug-free medium. Samples were taken at different times of post-incubation and γ-H2AX foci formation determined. Data are the mean ± s.d. from at least three independent experiments.