Figure 5
Over-expression with Bcl-2 or DN-caspase 9 does not rescue the simvastatin induces apoptosis in prostate tumor cells. (A) Bar graph showing caspase-9 activity in PC3 cells after 24 h treatment with simvastatin, docetaxel or a combination of both, compared to saline treated control. (B) Bar graph showing caspase-9 activity in PC3 cells over-expressing Bcl-2, DN-caspase 9 and both, compared to vector control in the presence and absence of 24h treatment with simvastatin. (C) Western blots showing protein expression of Bcl-2 and caspase-9 in PC3 cells over-expressing Bcl-2, DN-caspase 9 and both. (D) Bar graph showing apoptosis of PC3 cells over-expressing Bcl-2, DN-caspase 9 and both, compared to vector control in the presence and absence of 24h treatment with simvastatin. The data are presented as mean ± SD (n=4 of quadruplicate experiments).