Figure 2
From: Disruption of CTCF at the miR-125b1 locus in gynecological cancers
Analysis of the DNA methylation status in normal tissue and gynecological tumor samples. A- Analysis of DNA methylation by MS-PCR in several normal breast (NB), ovary (NO), cervix (NC) and peripheral blood lymphocytes (NL). Assessment of DNA methylation state in various gynecological primary tumors: Breast cancer (BC), ovarian cancer (OC) and cervical cancer (CC). B- Determination of the DNA methylation status using sodium bisulfite and sequencing in primary breast cancer samples and normal tissue samples. Black boxes represent methylated CpGs, and white boxes represent non-methylated CpGs. C- Expression analysis of miR-125b1 from primary breast cancer samples compared with normal breast tissue obtained during an aesthetic mammoplasty. **P < 0.001 Student's t-test.